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Campylobacter jejuni Antibody

This MAb is specific for a non-flagellar antigen of both C. jejuni Types 1 and 2. It reacts with a soluble excreted antigen in EIA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organisms. Campylobacter jejuni is a Gram-negative, microaerophilic, slender, flagellate, spiral bacterium. It is the major reported cause of bacterial foodborne infection in the United States and is also associated with Guillian-Barre syndrome. Campylobacteriosis is an infectious disease caused by bacteria of the genus Campylobacter. Most people who become ill with campylobacteriosis get diarrhea, cramping, abdominal pain, and fever within 2 to 5 days after exposure to the organism. The diarrhea may be bloody and can be accompanied by nausea and vomiting. The illness typically lasts 1 week. Some persons who are infected with Campylobacter don't have any symptoms at all. In persons with compromised immune systems, Campylobacter occasionally spreads to the bloodstream and causes a serious life threatening infection. The Campylobacter organism is actually a group of spiral shaped bacteria that can cause disease in humans and animals. Most human illness is caused by one species, called Campylobacter jejuni, but 1% of human Campylobacter cases are caused by other species.

Product Specifications

Reactivity

Bacteria

Immunogen

Total sonicate of Campylobacter jejuni was used as the immunogen for the Campylobacter jejuni antibody.

Clonality

Monoclonal

Clone

2E-10.

Conjugation

Unconjugated

Purification

PEG precipitation followed by dialysis in 1X PBS

Dilution

ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format), Immunofluorescence: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Campylobacter jejuni antibody should be determined by the researcher.

Tested Applications

ELISA, IF

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgM, kappa

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