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Hepatocyte Specific Antigen Antibody

MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.

Product Specifications

Reactivity

Human

Immunogen

The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.

Clonality

Monoclonal

Clone

HSA133

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation; Gastroenterology & Hepatology

Purification

Protein G affinity chromatography

Dilution

ELISA (For coating, purchase antibody without BSA)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.

Tested Applications

ELISA

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2b, kappa

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