Hepatocyte Specific Antigen Antibody
MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
Product Specifications
Reactivity
Human
Immunogen
The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
Clonality
Monoclonal
Clone
HSA133
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation; Gastroenterology & Hepatology
Purification
Protein G affinity chromatography
Dilution
ELISA (For coating, purchase antibody without BSA)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.
Tested Applications
ELISA
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa
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