What is the catalog number for Thymidylate Synthase Antibody Cocktail?

The catalog number for Thymidylate Synthase Antibody Cocktail is orb2639846.

What sizes are available for Thymidylate Synthase Antibody Cocktail?

Thymidylate Synthase Antibody Cocktail is available in the following size(s): 7 mL.

Is Thymidylate Synthase Antibody Cocktail in stock?

Thymidylate Synthase Antibody Cocktail is currently made to order / not held in immediate stock. Contact us for lead time.

Thymidylate Synthase Antibody Cocktail

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45) . TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Specifications

UniProt

P04818

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogens for this Thymidylate Synthase antibody cocktail.

Clonality

Monoclonal

Clone

TS106 + TMS715

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the Thymidylate Synthase antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.