What is the catalog number for Bcl-X Antibody?

The catalog number for Bcl-X Antibody is orb2639743.

What sizes are available for Bcl-X Antibody?

Bcl-X Antibody is available in the following size(s): 100 µg.

Is Bcl-X Antibody in stock?

Bcl-X Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Bcl-X Antibody

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

Product Specifications

UniProt

Q07817

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.

Clonality

Monoclonal

Clone

2H12

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.