What is the catalog number for Basic Cytokeratin Antibody?

The catalog number for Basic Cytokeratin Antibody is orb2639731.

What sizes are available for Basic Cytokeratin Antibody?

Basic Cytokeratin Antibody is available in the following size(s): 7 mL.

Is Basic Cytokeratin Antibody in stock?

Basic Cytokeratin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Basic Cytokeratin Antibody

This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

UniProt

Q01546

Reactivity

Human, Rat

Immunogen

Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.

Clonality

Monoclonal

Clone

KRTH/1076

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.