What is the catalog number for HCG Holo Antibody?

The catalog number for HCG Holo Antibody is orb2639603.

What sizes are available for HCG Holo Antibody?

HCG Holo Antibody is available in the following size(s): 100 µg.

Is HCG Holo Antibody in stock?

HCG Holo Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

HCG Holo Antibody

This mAb reacts only with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH) . HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Product Specifications

UniProt

P01215, P0DN86

Reactivity

Human

Immunogen

Purified hCG protein was used as the immunogen for the hCG Holo antibody.

Clonality

Monoclonal

Clone

HCGab/52

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the hCG Holo antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.