What is the catalog number for Napsin A Antibody?

The catalog number for Napsin A Antibody is orb2639449.

What sizes are available for Napsin A Antibody?

Napsin A Antibody is available in the following size(s): 100 µg.

Is Napsin A Antibody in stock?

Napsin A Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Napsin A Antibody

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Specifications

UniProt

O96009

Reactivity

Human

Immunogen

A recombinant fragment corresponding to amino acids 189-299 from the human protein was used as the immunogen for the Napsin A antibody.

Clonality

Monoclonal

Clone

NAPSA/1238

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Napsin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.