What is the catalog number for Carbonic Anhydrase 9 Antibody?

The catalog number for Carbonic Anhydrase 9 Antibody is orb2639371.

What sizes are available for Carbonic Anhydrase 9 Antibody?

Carbonic Anhydrase 9 Antibody is available in the following size(s): 100 µg.

Is Carbonic Anhydrase 9 Antibody in stock?

Carbonic Anhydrase 9 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Carbonic Anhydrase 9 Antibody

Recognizes a glycoprotein identified as carbonic anhydrase IX (CAIX/CA9) . Its epitope resides in the carbohydrate domain of CA9. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

UniProt

Q16790

Reactivity

Human

Immunogen

The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for the Carbonic Anhydrase 9 antibody.

Clonality

Monoclonal

Clone

PN-15

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Carbonic Anhydrase 9 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.