What is the catalog number for ZAP70 Antibody?

The catalog number for ZAP70 Antibody is orb2639359.

What sizes are available for ZAP70 Antibody?

ZAP70 Antibody is available in the following size(s): 100 µg.

Is ZAP70 Antibody in stock?

ZAP70 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

ZAP70 Antibody

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Product Specifications

UniProt

P43403

Reactivity

Human

Immunogen

Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP70 antibody.

Clonality

Monoclonal

Clone

SPM362

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the ZAP70 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.