What is the catalog number for VCAM-1 Antibody?

The catalog number for VCAM-1 Antibody is orb2639324.

What sizes are available for VCAM-1 Antibody?

VCAM-1 Antibody is available in the following size(s): 100 µg.

Is VCAM-1 Antibody in stock?

VCAM-1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

VCAM-1 Antibody

Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1), L1CAM and INCAM-100) . It is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM-1 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.

Product Specifications

UniProt

P19320

Reactivity

Human

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the VCAM-1 antibody.

Clonality

Monoclonal

Clone

1.4C3

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the VCAM-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.