What is the catalog number for Gp96 Antibody / GRP94 / HSP90B1?

The catalog number for Gp96 Antibody / GRP94 / HSP90B1 is orb2639298.

What sizes are available for Gp96 Antibody / GRP94 / HSP90B1?

Gp96 Antibody / GRP94 / HSP90B1 is available in the following size(s): 100 µg.

Is Gp96 Antibody / GRP94 / HSP90B1 in stock?

Gp96 Antibody / GRP94 / HSP90B1 is currently made to order / not held in immediate stock. Contact us for lead time.

Gp96 Antibody / GRP94 / HSP90B1

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96) . Gp96 shows a high degree of sequence homology with the heat shock protein 90 (hsp90) . This mAb is highly specific and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Gp96 has been briefly studied as a prognostic factor in breast cancer.

Product Specifications

UniProt

P14625

Reactivity

Human

Immunogen

Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the gp96 antibody.

Clonality

Monoclonal

Clone

SPM249

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the gp96 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.