What is the catalog number for TGF-alpha Antibody?

The catalog number for TGF-alpha Antibody is orb2639248.

What sizes are available for TGF-alpha Antibody?

TGF-alpha Antibody is available in the following size(s): 100 µg.

Is TGF-alpha Antibody in stock?

TGF-alpha Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

TGF-alpha Antibody

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Product Specifications

UniProt

P01135

Reactivity

Human

Immunogen

Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.

Clonality

Monoclonal

Clone

P/T1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the TGF-alpha antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.