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CD43 Antibody

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity) . During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity) . [UniProt]

Product Specifications

UniProt

P16150

Reactivity

Human

Immunogen

Immature pluripotent human leukemia K562 cells were used as the immunogen for the CD43 antibody.

Clonality

Monoclonal

Clone

Bra7G

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

PEG precipitation

Dilution

Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1) (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CD43 antibody should be determined by the researcher.1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at room temp2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgM, kappa

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