What is the catalog number for Cyclin D1 Antibody?

The catalog number for Cyclin D1 Antibody is orb2639135.

What sizes are available for Cyclin D1 Antibody?

Cyclin D1 Antibody is available in the following size(s): 100 µg.

Is Cyclin D1 Antibody in stock?

Cyclin D1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Cyclin D1 Antibody

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Product Specifications

UniProt

P24385

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.

Clonality

Monoclonal

Clone

CCND1/809

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/million cells, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.