What is the catalog number for PAX-7 Antibody?

The catalog number for PAX-7 Antibody is orb2639043.

What sizes are available for PAX-7 Antibody?

PAX-7 Antibody is available in the following size(s): 100 µg.

Is PAX-7 Antibody in stock?

PAX-7 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

PAX-7 Antibody

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .

Product Specifications

UniProt

P23759

Reactivity

Human

Immunogen

Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this PAX-7 antibody.

Clonality

Monoclonal

Clone

SPM613

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the PAX-7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.