What is the catalog number for CD146 Antibody / MCAM?

The catalog number for CD146 Antibody / MCAM is orb2638841.

What sizes are available for CD146 Antibody / MCAM?

CD146 Antibody / MCAM is available in the following size(s): 100 µg.

Is CD146 Antibody / MCAM in stock?

CD146 Antibody / MCAM is currently made to order / not held in immediate stock. Contact us for lead time.

CD146 Antibody / MCAM

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

UniProt

P43121

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the anti-CD146 antibody.

Clonality

Monoclonal

Clone

SPM620

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the anti-CD146 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.