What is the catalog number for Keratin 18 Antibody?

The catalog number for Keratin 18 Antibody is orb2638780.

What sizes are available for Keratin 18 Antibody?

Keratin 18 Antibody is available in the following size(s): 100 µg.

Is Keratin 18 Antibody in stock?

Keratin 18 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Keratin 18 Antibody

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Product Specifications

UniProt

P05783

Reactivity

Human, Mouse, Rat

Immunogen

A cytoskeletal preparation from A431 cells was used as the immunogen for the Keratin 18 antibody.

Clonality

Monoclonal

Clone

C-04 or Ks18.04

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Keratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.