What is the catalog number for Keratin 7 Antibody Cocktail?

The catalog number for Keratin 7 Antibody Cocktail is orb2638737.

What sizes are available for Keratin 7 Antibody Cocktail?

Keratin 7 Antibody Cocktail is available in the following size(s): 7 mL.

Is Keratin 7 Antibody Cocktail in stock?

Keratin 7 Antibody Cocktail is currently made to order / not held in immediate stock. Contact us for lead time.

Keratin 7 Antibody Cocktail

It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .

Product Specifications

UniProt

P08729

Reactivity

Human

Immunogen

Recombinant full-length human KRT7 protein was used as the immunogen for the Keratin 7 antibody cocktail.

Clonality

Monoclonal

Clone

KRT7/760 + KRT7/903

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Keratin 7 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.