HLA-DR Antibody (MHC II)
HLA-DR binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]
Product Specifications
UniProt
P01911
Reactivity
Human
Immunogen
Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DR antibody.
Clonality
Monoclonal
Clone
LN3
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation; Cell Biology
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the HLA-DR antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG2b, kappa
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