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SSEA-1 Antibody Cocktail

SSEA-1/CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, it is expressed in Reed-Sternberg cells and some epithelial cells. SSEA-1/CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Product Specifications

UniProt

P22083

Reactivity

Human, Mouse, Rat

Immunogen

Purified human neutrophils (FUT4/815) and Myelomonocytic leukemia cells (BRA-4F1) were used as the immunogen for the SSEA-1 antibody cocktail.

Clonality

Monoclonal

Clone

FUT4/815 + BRA-4F1

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

PEG precipitation

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the SSEA-1 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgM, kappa

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