What is the catalog number for Mitochondria Marker Antibody?

The catalog number for Mitochondria Marker Antibody is orb2638082.

What sizes are available for Mitochondria Marker Antibody?

Mitochondria Marker Antibody is available in the following size(s): 7 mL.

Is Mitochondria Marker Antibody in stock?

Mitochondria Marker Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Mitochondria Marker Antibody

MAb MTC754 recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Specifications

Reactivity

Human

Immunogen

The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.

Clonality

Monoclonal

Clone

MTC754

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondria marker antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues is enhanced by boiling sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.