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IL-6 Antibody

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Product Specifications

Reactivity

Mouse

Immunogen

Recombinant mouse IL-6 protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

MP5-20F3

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G purified IL-6 antibody

Dilution

Neutralization Studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IL-6 antibody to be titered up or down for optimal performance.

Tested Applications

FACS, IF, NeA

Host or Source

Rat

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Rat IgG1, kappa

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