What is the catalog number for BrdU Antibody?

The catalog number for BrdU Antibody is orb2638028.

What sizes are available for BrdU Antibody?

BrdU Antibody is available in the following size(s): 7 mL.

Is BrdU Antibody in stock?

BrdU Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

BrdU Antibody

This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

Reactivity

All

Immunogen

BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

BRD494

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.