CLIC4 Antibody

Goat polyclonal antibody to CLIC4

Product Specifications

Product Name Alternative

Anti CLIC4 antibody, anti chloride intracellular channel 4 antibody, anti CLIC4L antibody, anti DKFZP566G223 antibody, anti FLJ38640 antibody, anti H1 antibody, anti huH1 antibody, anti p64H1 antibody, anti chloride intracellular channel 4 like antibody

Reactivity

Human, Mouse

Target

CLIC4

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NGLKEEDKEPLIE

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

28.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 30kDa band observed in human kidney and human placenta lysates (calculated MW of 28.8kDa according to NP_039234.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar;276 (3 Pt 2) :F398-408.; PMID: 10070163) . Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5ug/ml with overnight incubation.

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.