CD16 Antibody
Recognizes a protein of 50-65 kDa, identified as CD16 (Workshop IV; Code N39) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11) . CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI) -anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.
Product Specifications
UniProt
P08637
Reactivity
Human
Immunogen
Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.
Clonality
Monoclonal
Clone
HuNK2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD16 antibody should be determined by the researcher.
Tested Applications
FACS, Functional Studies, IF
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2a, kappa
Frequently Asked Questions
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