What is the catalog number for PGP9.5 Antibody / UchL1?

The catalog number for PGP9.5 Antibody / UchL1 is orb2637990.

What sizes are available for PGP9.5 Antibody / UchL1?

PGP9.5 Antibody / UchL1 is available in the following size(s): 100 µg.

Is PGP9.5 Antibody / UchL1 in stock?

PGP9.5 Antibody / UchL1 is currently made to order / not held in immediate stock. Contact us for lead time.

PGP9.5 Antibody / UchL1

This antibody specifically reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1) . Initially, expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. PGP9.5 antibody has been used to show the protein to be a useful prognostic indicator of colorectal, pancreatic and gall bladder cancer. A mutation in the gene is believed to cause a form of Parkinson's disease.

Product Specifications

Reactivity

Human, Mouse, Rat

Immunogen

Native protein from brain was used as the immunogen for this PGP9.5 antibody.

Clonality

Monoclonal

Clone

31A3

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.