TGF alpha Antibody
TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Product Specifications
Reactivity
Human, Mouse, Rat
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
MF9
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
ELISA: 2-4ug/ml (order BSA/azide-free format), Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
ELISA, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa
Frequently Asked Questions
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