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Progesterone Receptor Antibody

This antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa) . Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.

Product Specifications

Reactivity

Human

Immunogen

Recombinant human Progesterone Receptor protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

PR501

Conjugation

Unconjugated

Field of Research

Pharmacology & Drug Discovery

Purification

Protein G affinity chromatography

Dilution

Western blot: 0.5-1ug/ml for 2 hr at RT, Immunohistochemistry (FFPE) : 0.5-1ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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