What is the catalog number for MUC5AC Antibody / Mucin-5AC?

The catalog number for MUC5AC Antibody / Mucin-5AC is orb2637855.

What sizes are available for MUC5AC Antibody / Mucin-5AC?

MUC5AC Antibody / Mucin-5AC is available in the following size(s): 100 µg.

Is MUC5AC Antibody / Mucin-5AC in stock?

MUC5AC Antibody / Mucin-5AC is currently made to order / not held in immediate stock. Contact us for lead time.

MUC5AC Antibody / Mucin-5AC

This antibody recognizes the peptide core of gastric mucin M1 (>1,000kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Product Specifications

Reactivity

Human, Mouse, Rat

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient was used as the immunogen for this MUC5AC antibody.

Clonality

Monoclonal

Clone

45M1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.