What is the catalog number for MUC2 Antibody / Mucin-2?

The catalog number for MUC2 Antibody / Mucin-2 is orb2637852.

What sizes are available for MUC2 Antibody / Mucin-2?

MUC2 Antibody / Mucin-2 is available in the following size(s): 100 µg.

Is MUC2 Antibody / Mucin-2 in stock?

MUC2 Antibody / Mucin-2 is currently made to order / not held in immediate stock. Contact us for lead time.

MUC2 Antibody / Mucin-2

This antibody recognizes a single glycoprotein of 520kDa, identified as MUC2 (Mucin-2) . The MUC2 antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine and colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Product Specifications

Reactivity

Human

Immunogen

Amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT, from human MUC2 / Mucin-2 protein, were used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

CCP58

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.