Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

EpCAM Antibody

Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

Reactivity

Human

Immunogen

MCF-7 human breast cancer cells were used as the immunogen for this EpCAM antibody.

Clonality

Monoclonal

Clone

323/A3

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG1, kappa

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Mycoprep 75Ml Bottles
240862 10 / PCK

Mycoprep 75Ml Bottles

Sign In for Pricing
View Details
SLC4A7 Polyclonal Antibody
MBS2519128-01 0.02 mL

SLC4A7 Polyclonal Antibody

Sign In for Pricing
View Details
SLC4A7 Polyclonal Antibody
MBS2519128-02 0.06 mL

SLC4A7 Polyclonal Antibody

Sign In for Pricing
View Details
SLC4A7 Polyclonal Antibody
MBS2519128-03 0.12 mL

SLC4A7 Polyclonal Antibody

Sign In for Pricing
View Details
SLC4A7 Polyclonal Antibody
MBS2519128-04 0.2 mL

SLC4A7 Polyclonal Antibody

Sign In for Pricing
View Details
SLC4A7 Polyclonal Antibody
MBS2519128-05 5x 0.2 mL

SLC4A7 Polyclonal Antibody

Sign In for Pricing
View Details