What is the catalog number for CD50 Antibody?

The catalog number for CD50 Antibody is orb2637744.

What sizes are available for CD50 Antibody?

CD50 Antibody is available in the following size(s): 100 µg.

Is CD50 Antibody in stock?

CD50 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

CD50 Antibody

This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI) . It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.

Product Specifications

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for this CD50 antibody.

Clonality

Monoclonal

Clone

186-2G9

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (order BSA/sodium azide-free format), Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.