What is the catalog number for GFAP Antibody?

The catalog number for GFAP Antibody is orb2637731.

What sizes are available for GFAP Antibody?

GFAP Antibody is available in the following size(s): 100 µg.

Is GFAP Antibody in stock?

GFAP Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

GFAP Antibody

This antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

Reactivity

Human, Mouse, Rat

Immunogen

GFAP isolated from pig spinal cord was used as the immunogen.

Clonality

Monoclonal

Clone

GA-5

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/million, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GFAP antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.