What is the catalog number for MART-1 Antibody Cocktail?

The catalog number for MART-1 Antibody Cocktail is orb2637716.

What sizes are available for MART-1 Antibody Cocktail?

MART-1 Antibody Cocktail is available in the following size(s): 7 mL.

Is MART-1 Antibody Cocktail in stock?

MART-1 Antibody Cocktail is currently made to order / not held in immediate stock. Contact us for lead time.

MART-1 Antibody Cocktail

This antibody cocktail recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody cocktail does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Product Specifications

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (A103; M2-7C10; M2-9E3) was used as the immunogen for this MART-1 antibody cocktail.

Clonality

Monoclonal

Clone

A103 + M2-7C10 + M2-9E3

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.