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ER Antibody / Estrogen Receptor alpha

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.

Product Specifications

Reactivity

Human

Immunogen

Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.

Clonality

Monoclonal

Clone

ER506

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Western blot: 2-4ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml (1), Immunofluorescence: 1-3ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.1. FFPE testing requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and cooling at RT for 20 minutes.

Tested Applications

FACS, IF, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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