What is the catalog number for PSA Antibody / Prostate Specific Antigen?

The catalog number for PSA Antibody / Prostate Specific Antigen is orb2637512.

What sizes are available for PSA Antibody / Prostate Specific Antigen?

PSA Antibody / Prostate Specific Antigen is available in the following size(s): 7 mL.

Is PSA Antibody / Prostate Specific Antigen in stock?

PSA Antibody / Prostate Specific Antigen is currently made to order / not held in immediate stock. Contact us for lead time.

PSA Antibody / Prostate Specific Antigen

This antibody recognizes a single protein of 33-34kDa, identified as the Prostate specific antigen. This PSA antibody is highly specific and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. Prostate specific antigen is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT) . A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign hyperplasia.

Product Specifications

Reactivity

Human

Immunogen

Prostate Specific Antigen from human sperm plasma was used as the antigen for this PSA antibody.

Clonality

Monoclonal

Clone

A67-B/E3

Conjugation

Unconjugated

Field of Research

Cancer Biology; Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.