Biotin Antibody
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Product Specifications
Reactivity
All
Immunogen
Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Clonality
Monoclonal
Clone
BTN399
Conjugation
Unconjugated
Purification
Protein A/G affinity
Dilution
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-2ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Biotin antibody should be determined by the researcher.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa
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