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HA Tag Antibody

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Product Specifications

Immunogen

Amino acids 98-106 (YPYDVPDYA) were used as the immunogen for the HA Tag antibody.

Clonality

Monoclonal

Clone

HA/279

Conjugation

Unconjugated

Purification

Protein A/G affinity

Dilution

ELISA (order BSA-free format for coating), Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate), Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the HA Tag antibody should be determined by the researcher.

Tested Applications

ELISA, FACS, IF, IHC-P, IP, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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