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Recombinant Cytokeratin 20 Antibody / CK20

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.

Product Specifications

UniProt

P35900

Reactivity

Human

Immunogen

A portion of amino acids 196-323 was used as the immunogen for the recombinant Cytokeratin 20 antibody.

Clonality

Recombinant

Clone

RKRT20/6536

Conjugation

Unconjugated

Purification

Protein A/G affinity

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml, Western blot: 2-4ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant Cytokeratin 20 antibody should be determined by the researcher.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2a, kappa

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