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CK-BB Antibody / Creatine Phosphokinase-B / CKB

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Specifications

UniProt

P12277

Reactivity

Guinea pig, Hamster, Human, Mouse, Rat

Immunogen

Recombinant human full-length CKB protein was used as the immunogen for the CKB antibody.

Clonality

Monoclonal

Clone

CKBB/6871

Conjugation

Unconjugated

Field of Research

Pharmacology & Drug Discovery

Purification

Protein A/G affinity

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CK-BB antibody should be determined by the researcher.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2b, kappa

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