RPA32 Antibody / RPA2 / Replication Protein A2
Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) . RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively) . It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Product Specifications
UniProt
P15927
Reactivity
Human
Immunogen
Recombinant human full-length Replication Protein A2 protein was used as the immunogen for the RPA32 antibody.
Clonality
Monoclonal
Clone
RPA2/4774
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Protein A/G affinity
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the RPA32 antibody should be determined by the researcher.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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