Recombinant VIM Antibody / Vimentin
When used in conjunction with anti-keratin staining, anti-vimentin staining is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma.
Product Specifications
UniProt
P08670
Reactivity
Human
Immunogen
A portion of amino acids 366-466 was used as the immunogen for the recombinant VIM antibody.
Clonality
Recombinant
Clone
RVIM/6914
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml, Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant VIM antibody should be determined by the researcher.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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