Recombinant Human Nuclear Antigen Antibody
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Product Specifications
Reactivity
Human
Immunogen
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.
Clonality
Recombinant
Clone
235-1R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A/G affinity
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant Human Nuclear Antigen antibody should be determined by the researcher.
Tested Applications
FACS, IF
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa
Frequently Asked Questions
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