CD50 Antibody / ICAM-3
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI) . CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.
Product Specifications
UniProt
P32942
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the CD50 antibody.
Clonality
Monoclonal
Clone
CG106
Conjugation
Unconjugated
Field of Research
Neuroscience; Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Blocks binding of CD11a/LFA-1 to CD50/ICAM-3 (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD50 antibody should be determined by the researcher.
Tested Applications
Blocking, FACS, IF
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2a, kappa
Frequently Asked Questions
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