Estrogen Receptor alpha Antibody
This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
Product Specifications
UniProt
P03372
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 129-312) was used as the immunogen for this Estrogen Receptor alpha antibody.
Clonality
Monoclonal
Clone
ESR1/3559
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunohistochemistry (FFPE) : 1-2ug/ml, Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.
Tested Applications
FACS, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa
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