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Clathrin Heavy Chain Antibody

Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.

Product Specifications

UniProt

Q00610, P53675

Reactivity

Human

Immunogen

Recombinant full-length human Clathrin Heavy Chain protein was used as the immunogen for this Clathrin Heavy Chain antibody.

Clonality

Monoclonal

Clone

CHC/1432

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunofluorescence: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.

Tested Applications

IF

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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