Cytokeratin 5/6/18 Antibody
It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
Product Specifications
Reactivity
Human
Immunogen
The detergent-insoluble fraction of psoriatic human epidermis was used as the immunogen for the Cytokeratin 5/6/18 antibody.
Clonality
Monoclonal
Clone
LP34
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 0.1-0.2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Cytokeratin 5/6/18 antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa
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