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CD47 Antibody / IAP

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Product Specifications

UniProt

Q08722

Reactivity

Human

Immunogen

Amino acids 18-135 from the human protein were used as the immunogen for the CD47 antibody.

Clonality

Monoclonal

Clone

CD47/2937

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation; Cell Biology

Purification

Protein G affinity chromatography

Dilution

ELISA: order BSA/sodium azide-free format for coating, Western blot: 1-2ug/ml for 60 min at RT, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CD47 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

ELISA, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2c, kappa

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