ACTN2 Antibody / Sarcomeric Alpha Actinin / Alpha Actinin 2
The ACTN2 gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.
Product Specifications
UniProt
P35609
Reactivity
Human
Immunogen
Amino acids 557-692 from the human protein were used as the immunogen for this ACTN2 antibody.
Clonality
Monoclonal
Clone
SAMA-1
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the ACTN2 antibody for each application should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa
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